6E0W

Crystal structure of the colanidase tailspike protein gp150 of Phage Phi92 complexed with one repeating unit of colanic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-03-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9919
Spacegroup name	H 3
Unit cell lengths	117.623, 117.623, 308.282
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	61.461 - 1.803
R-factor	0.1534
Rwork	0.153
R-free	0.17650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4RMX
RMSD bond length	0.015
RMSD bond angle	1.172
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((dev_3092: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.000	1.910
High resolution limit [Å]	1.800	1.800
Rmerge	0.076	0.539
Rmeas	0.096	0.491
Number of reflections	281287	44765
<I/σ(I)>	7.47	1.71
Completeness [%]	95.8	94.3
Redundancy	2.6	2.4
CC(1/2)	0.991	0.848

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	10% PEG 8000, 0.2M SrCl2, 0.1M MES pH 6.0, 10mg/ml