6E0T
C-terminal domain of Fission Yeast OFD1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-12 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.54184 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 81.085, 106.717, 64.649 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.091 - 2.020 |
| R-factor | 0.2024 |
| Rwork | 0.200 |
| R-free | 0.24370 |
| Structure solution method | MIR |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.916 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.091 | 2.070 |
| High resolution limit [Å] | 2.020 | 2.030 |
| Number of reflections | 18232 | 756 |
| <I/σ(I)> | 28 | |
| Completeness [%] | 96.9 | 82.4 |
| Redundancy | 6.8 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 1.2 M LiSo4, 0.1 M HEPES |
