6E0M
Structure of Elizabethkingia meningoseptica CdnE cyclic dinucleotide synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2017-10-18 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.195, 58.231, 99.446 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.804 - 1.520 |
| R-factor | 0.1409 |
| Rwork | 0.140 |
| R-free | 0.17620 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.818 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.580 | 1.540 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rpim | 0.033 | 0.623 |
| Number of reflections | 52028 | 2474 |
| <I/σ(I)> | 13.6 | 1.4 |
| Completeness [%] | 99.8 | 96.1 |
| Redundancy | 12.9 | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 16% PEG-5000 MME, 21 mM sodium citrate pH 7.0, 100 mM HEPES-KOH pH 7.5 |
