6E0L
Structure of Rhodothermus marinus CdnE c-UMP-AMP synthase with Apcpp and Upnpp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2017-11-15 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97910 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.647, 65.652, 88.855 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.920 - 2.250 |
| R-factor | 0.1836 |
| Rwork | 0.182 |
| R-free | 0.21660 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.091 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.920 | 2.320 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rpim | 0.068 | 0.304 |
| Number of reflections | 14799 | 1263 |
| <I/σ(I)> | 9.8 | 3 |
| Completeness [%] | 99.1 | 99.1 |
| Redundancy | 5.3 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 24% PEG-3350, 240 mM sodium malonate |
