6E0A

Crystal Structure of Helicobacter pylori TlpA Chemoreceptor Ligand Binding Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-01-30
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	72.779, 59.468, 77.723
Unit cell angles	90.00, 114.09, 90.00

Refinement procedure

Resolution	33.219 - 2.430
R-factor	0.1998
Rwork	0.197
R-free	0.25660
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.480
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.000	2.470
High resolution limit [Å]	2.430	2.430
Rmerge	0.038	0.177
Rpim	0.023	0.132
Number of reflections	83485
<I/σ(I)>	31.3	2.8
Completeness [%]	99.7	95.9
Redundancy	3.6
CC(1/2)	1.000	0.940

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	14.4% PEG 8000, 80mM NaCacodylate pH 6.5, 0.16M ZnOAc, with 20% glycerol as a cryoprotectant.