6DZ1
Studies of Ion Transport in K+ Channels
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I23 |
Synchrotron site | Diamond |
Beamline | I23 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-01 |
Detector | DECTRIS PILATUS 300K |
Wavelength(s) | 3.35 |
Spacegroup name | I 4 |
Unit cell lengths | 68.070, 68.070, 89.380 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.154 - 2.260 |
R-factor | 0.1799 |
Rwork | 0.178 |
R-free | 0.21120 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.087 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELXDE |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.150 | 2.330 |
High resolution limit [Å] | 2.260 | 2.260 |
Rmerge | 0.037 | 0.129 |
Rpim | 0.019 | 0.082 |
Number of reflections | 18417 | |
<I/σ(I)> | 33.4 | 10.7 |
Completeness [%] | 97.8 | 93.4 |
Redundancy | 8.2 | 6.3 |
CC(1/2) | 0.994 | 0.987 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | Prepared by mixing equal volumes of protein solution in buffer (20mM Tris:HCl pH 7.8, 100mM KCl, and 4mM Anagrade DM) with well solution (72.5%MPD, 100mM KCl, and 100mM MES pH 6) |