6DYR

C-terminal condensation domain of Ebony in complex with Carcinine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-10-17
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	44.520, 56.050, 49.730
Unit cell angles	90.00, 112.91, 90.00

Refinement procedure

Resolution	45.810 - 2.450
R-factor	0.1861
Rwork	0.184
R-free	0.22970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6dym
RMSD bond length	0.003
RMSD bond angle	0.501
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHENIX (1.13_2998)
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.810	2.510
High resolution limit [Å]	2.450	2.450
Number of reflections	8386
<I/σ(I)>	8
Completeness [%]	99.9
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	HEPES, PEG400, CaCl2