6DYN

C-terminal condensation domain of Ebony in complex with Histamine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-02-08
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	44.580, 57.470, 50.130
Unit cell angles	90.00, 113.23, 90.00

Refinement procedure

Resolution	40.970 - 2.102
Rwork	0.180
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6dym
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998:000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.970	2.160
High resolution limit [Å]	2.100	2.100
Rpim	0.050	0.400
Number of reflections	13655
<I/σ(I)>	11.9	2
Completeness [%]	99.6
Redundancy	4.4
CC(1/2)		0.630

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	HEPES, PEG 400, CaCl2