6DYM

C-terminal condensation domain of Ebony

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	44.958, 56.486, 50.523
Unit cell angles	90.00, 113.32, 90.00

Refinement procedure

Resolution	46.390 - 2.020
R-factor	0.178
Rwork	0.177
R-free	0.19830
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.626
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX ((1.13-2998-000))
Refinement software	PHENIX ((1.13-2998-000))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.390	2.070
High resolution limit [Å]	2.020	2.020
Number of reflections	15308
<I/σ(I)>	7.9
Completeness [%]	99.4
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	PEG 400, HEPES, CaCl2