6DY9
Crystal structure of double-stranded DNA GGGATCCC; crystals grown in Zn2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-05-29 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9786 |
Spacegroup name | P 61 |
Unit cell lengths | 46.221, 46.221, 43.426 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.000 - 2.300 |
R-factor | 0.2545 |
Rwork | 0.253 |
R-free | 0.27582 |
Structure solution method | SAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.738 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.250 |
High resolution limit [Å] | 2.210 | 2.210 |
Rmerge | 0.030 | 0.780 |
Number of reflections | 2702 | 128 |
<I/σ(I)> | 55 | |
Completeness [%] | 99.1 | 100 |
Redundancy | 6.1 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 294 | 1 mM ds DNA with 5 mM zinc acetate and 2 mM spermine was incubated against 55% (v/v) 2-methyl-2,4-pentanediol (MPD) |