6DY5
Crystal structure of double-stranded DNA AGGGATCCCT in complex with Zn2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-12 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 25.567, 42.451, 49.212 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.260 |
| R-factor | 0.21649 |
| Rwork | 0.214 |
| R-free | 0.25374 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.146 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.270 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.060 | 0.620 |
| Number of reflections | 14675 | 705 |
| <I/σ(I)> | 54 | |
| Completeness [%] | 97.3 | 94.1 |
| Redundancy | 6.8 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 1 mM ds DNA containing 5 mM zinc acetate, 2 mM spermine and 50 mM NaCl, incubated against 55% (v/v) 2-methyl-2,4-pentanediol (MPD) |
