6DY1

Rabbit N-acylethanolamine-hydrolyzing acid amidase (NAAA) with fatty acid (myristate), in presence of Triton X-100

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-17
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.98
Spacegroup name	P 43 3 2
Unit cell lengths	159.351, 159.351, 159.351
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.001 - 2.998
R-factor	0.198
Rwork	0.194
R-free	0.23310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5u7z
RMSD bond length	0.004
RMSD bond angle	0.654
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.110
High resolution limit [Å]	2.998	2.998
Number of reflections	14498
<I/σ(I)>	43
Completeness [%]	100.0
Redundancy	73.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	1.1 M (NH4)2SO4 with 0.1 M sodium acetate pH 4.5