6DXY

Murine N-acylethanolamine-hydrolyzing acid amidase (NAAA)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-04
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.98
Spacegroup name	H 3
Unit cell lengths	202.205, 202.205, 45.596
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.441 - 1.851
R-factor	0.1684
Rwork	0.167
R-free	0.19160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5u7z
RMSD bond length	0.004
RMSD bond angle	0.750
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.920
High resolution limit [Å]	1.851	1.851
Number of reflections	58028
<I/σ(I)>	16.5
Completeness [%]	98.0
Redundancy	8.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.2 M NaCl, 0.1 M BIS-TRIS pH 5 and 20 % PEG 3350