6DX5
Crystal structure of the viral OTU domain protease from Farallon virus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-01 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 70.276, 70.276, 176.258 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.941 - 2.224 |
R-factor | 0.1651 |
Rwork | 0.163 |
R-free | 0.20250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4hxd |
RMSD bond length | 0.015 |
RMSD bond angle | 1.228 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.260 |
High resolution limit [Å] | 2.220 | 2.220 |
Number of reflections | 22465 | 2182 |
<I/σ(I)> | 18.9 | 2.8 |
Completeness [%] | 99.6 | |
Redundancy | 6.9 | |
CC(1/2) | 0.990 | 0.890 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.3 M magnesium chloride, 0.1 M MES pH 6.5, 8% PEG 4000 |