6DVU

Structure of the Monoclinic-1 (Monocl-1) Crystal Form of Human Apolipoprotein C1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1992-06-15
Detector	SDMS
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	29.541, 46.739, 33.880
Unit cell angles	90.00, 95.16, 90.00

Refinement procedure

Resolution	33.000 - 1.800
R-factor	0.22426
Rwork	0.221
R-free	0.27626
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rop
RMSD bond length	0.009
RMSD bond angle	1.049
Data reduction software	UCSD-system
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	1.950
High resolution limit [Å]	1.800	1.800
Rmerge	0.094	0.457
Rmeas	0.114	0.721
Rpim	0.055	0.315
Number of reflections	7315	613
<I/σ(I)>	6.7	3.2
Completeness [%]	85.2	76
Redundancy	7.2	4.5
CC(1/2)	0.993	0.640

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	298	Crystallized in Cryschem sitting drop plates with reservoirs of 16%-18% 2-methyl-2,4-pentanediol (MPD), 0.1 M sodium acetate and 0.25% octyl-beta-s-1-thioglucopyanoside. The drops were composed of equal amounts of 8 mg/ml protein in 0.02 ammonium bicarbonate and reservoir.