6DVC
Crystal structure of Mycobacterium tuberculosis transcription initiation complex(ECF sigma factor L) containing 5nt RNA with 6nt spacer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 146.319, 161.519, 240.586 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 92.922 - 3.300 |
| R-factor | 0.1994 |
| Rwork | 0.198 |
| R-free | 0.23460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.468 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless (0.5.21) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 98.720 | 3.130 |
| High resolution limit [Å] | 3.080 | 3.080 |
| Rmerge | 0.179 | 2.567 |
| Number of reflections | 98083 | |
| <I/σ(I)> | 5.9 | |
| Completeness [%] | 93.0 | 90.2 |
| Redundancy | 4.1 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 100 mM sodium citrate tribasic dihydrate, pH 6.0, 200 mM sodium acetate, and 10% PEG4000 |
