6DV9
Crystal structure of Mycobacterium tuberculosis transcription initiation complex(ECF sigma factor L) containing 5nt RNA with 4nt spacer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-05-12 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 143.270, 161.440, 237.690 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 91.471 - 3.800 |
R-factor | 0.1868 |
Rwork | 0.184 |
R-free | 0.23200 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.489 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless (0.5.21) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 97.690 | 3.910 |
High resolution limit [Å] | 3.800 | 3.800 |
Rmerge | 0.330 | 1.967 |
Number of reflections | 53020 | |
<I/σ(I)> | 8.6 | |
Completeness [%] | 96.6 | 90.5 |
Redundancy | 10.7 | 9.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 100 mM sodium citrate tribasic dihydrate, pH 5.5, 200 mM sodium acetate, and 10% PEG4000 |