6DU5
Crystal structure of hMettl16 catalytic domain in complex with MAT2A 3'UTR hairpin 6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-09-01 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97934 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 134.918, 134.918, 76.208 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.731 - 3.006 |
R-factor | 0.2485 |
Rwork | 0.248 |
R-free | 0.26480 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.794 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.074 | 1.226 |
Number of reflections | 14533 | 1417 |
<I/σ(I)> | 46 | 2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 25 | 22 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1M Tris (pH 8.5), 20% glycerol, and 19% polyethylene (PEG) 10000 |