6DU2

Structure of Scp1 D96N bound to REST-pS861/4 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	80
Detector technology	PIXEL
Collection date	2015-02-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97648
Spacegroup name	C 1 2 1
Unit cell lengths	125.335, 78.568, 62.883
Unit cell angles	90.00, 112.13, 90.00

Refinement procedure

Resolution	39.460 - 2.500
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.540
High resolution limit [Å]	2.500	6.780	2.500
Rmerge	0.104	0.049	0.486
Total number of observations	70073
Number of reflections	19287	1021	815
<I/σ(I)>	6.5
Completeness [%]	98.5	99.9	84.3
Redundancy	3.6	3.7	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	30% PEG 3350 0.2 M Magnesium Acetate