6DTS
Maltotetraose bound T. maritima MalE2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2018-06-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 35.441, 56.123, 88.322 |
| Unit cell angles | 89.58, 84.63, 89.67 |
Refinement procedure
| Resolution | 31.737 - 1.500 |
| R-factor | 0.1696 |
| Rwork | 0.169 |
| R-free | 0.19070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fnc |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.036 | 0.125 |
| Number of reflections | 98262 | 7822 |
| <I/σ(I)> | 7.1 | |
| Completeness [%] | 90.3 | |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 23% PEG 3350, 0.3M sodium acetate and 0.1M bis-Tris pH 5.5 |
