6DTL
Mitogen-activated protein kinase 6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2016-06-26 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 151.589, 151.589, 85.991 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.977 - 2.753 |
R-factor | 0.209 |
Rwork | 0.205 |
R-free | 0.25640 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.680 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.750 | 2.750 |
Number of reflections | 29831 | |
<I/σ(I)> | 17.53 | |
Completeness [%] | 100.0 | |
Redundancy | 11.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | sodium citrate, PEG8000 |