6DTH

Crystal structure of Haemophilus influenzae OppA complex with RPPGFSPFR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-14
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0782
Spacegroup name	P 21 21 21
Unit cell lengths	47.865, 92.887, 108.119
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.440 - 1.960
R-factor	0.1938
Rwork	0.191
R-free	0.25400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.262
Data reduction software	XDS (Jan 26, 201)
Data scaling software	Aimless (0.6.2)
Phasing software	BALBES (1.0.0)
Refinement software	REFMAC (5.8.0222)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.440	2.010
High resolution limit [Å]	1.960	1.960
Rmerge	0.084	0.717
Number of reflections	35422	2481
<I/σ(I)>	11.6	2.4
Completeness [%]	99.9	100
Redundancy	8.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.1M sodium acetate pH 4.6, 2.3M ammonium sulfate