6DTD

High-resolution crystal structure of Cas13b from Prevotella buccae

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	93.15
Detector technology	PIXEL
Collection date	2018-05-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.03320
Spacegroup name	P 21 21 21
Unit cell lengths	90.821, 124.646, 140.728
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.654 - 1.650
R-factor	0.1548
Rwork	0.153
R-free	0.18500
Structure solution method	MAD
Data scaling software	XDS
Phasing software	AutoSol
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.654	46.654	1.380
High resolution limit [Å]	1.340	6.000	1.340
Rmerge	0.127	0.071
Rmeas	0.132	0.074
Total number of observations	3076708
Number of reflections	209453	4296	2464
<I/σ(I)>	7.57	31.75
Completeness [%]	80.5	100	9.5
Redundancy	10.782	12.941	1.354
CC(1/2)	0.998	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293.15	salt, PEG