6DT1
Crystal structure of the ligase from bacteriophage T4 complexed with DNA intermediate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 |
| Unit cell lengths | 63.526, 67.429, 114.635 |
| Unit cell angles | 88.78, 80.94, 62.97 |
Refinement procedure
| Resolution | 113.021 - 2.750 |
| R-factor | 0.1723 |
| Rwork | 0.170 |
| R-free | 0.21910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x9n |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.796 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14rc1_3177: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 113.572 | 2.850 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.078 | 0.540 |
| Number of reflections | 42792 | 4259 |
| <I/σ(I)> | 9.3 | 1.4 |
| Completeness [%] | 98.6 | 98.1 |
| Redundancy | 2 | 2 |
| CC(1/2) | 0.988 | 0.622 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG3350 |
