6DSY

Bst DNA polymerase I post-chemistry (n+1) structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2017-08-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	88.199, 93.751, 105.846
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.087 - 1.980
R-factor	0.2044
Rwork	0.203
R-free	0.25480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l3t
RMSD bond length	0.007
RMSD bond angle	0.883
Data reduction software	autoXDS
Data scaling software	autoXDS
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.090	2.051
High resolution limit [Å]	1.980	1.980
Rmerge	0.708	1.389
Number of reflections	61577	6059
<I/σ(I)>	43.97
Completeness [%]	97.0
Redundancy	12.9
CC(1/2)	0.758	0.524

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	50% ammonium sulfate, 0.1M MES, 2% MPD