6DSQ
Re-refinement of P. falciparum orotidine 5'-monophosphate decarboxylase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2005-08-29 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.977 |
Spacegroup name | H 3 |
Unit cell lengths | 201.812, 201.812, 44.026 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.318 - 2.700 |
R-factor | 0.2004 |
Rwork | 0.195 |
R-free | 0.25090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3n3m |
RMSD bond length | 0.008 |
RMSD bond angle | 0.971 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.700 |
Number of reflections | 18313 |
<I/σ(I)> | 4 |
Completeness [%] | 99.6 |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 23% PEG 3000, pH 9.5 |