6DRU
Xylosidase from Aspergillus niger
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-17 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 146.132, 146.132, 220.081 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.915 - 2.700 |
R-factor | 0.1818 |
Rwork | 0.181 |
R-free | 0.21250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xvg |
RMSD bond length | 0.002 |
RMSD bond angle | 0.531 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.915 | 47.915 | 2.390 |
High resolution limit [Å] | 2.260 | 6.710 | 2.260 |
Rmerge | 0.525 | 0.074 | |
Rmeas | 0.541 | 0.076 | |
Total number of observations | 2108169 | ||
Number of reflections | 124948 | 5163 | 17700 |
<I/σ(I)> | 5.71 | 38.13 | 0.03 |
Completeness [%] | 97.5 | 99.6 | 86.3 |
Redundancy | 16.872 | 20.279 | 3.761 |
CC(1/2) | 0.983 | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 293.15 | 5% v/v 2-Propanol, 0.1 M Citric acid pH 3.5, 6% w/v Polyethylene glycol 20,000 |