6DRR
Crystal structure of Cj0485 dehydrogenase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-002 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-02-20 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.54187 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 100.659, 108.780, 105.638 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.868 - 1.599 |
R-factor | 0.176 |
Rwork | 0.174 |
R-free | 0.20550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4gvx |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 73.880 | 1.630 |
High resolution limit [Å] | 1.599 | 1.600 |
Rmerge | 0.071 | 0.328 |
Rpim | 0.028 | 0.225 |
Number of reflections | 76135 | |
<I/σ(I)> | 22.9 | 2.6 |
Completeness [%] | 99.7 | 99.1 |
Redundancy | 4.7 | 2.8 |
CC(1/2) | 0.997 | 0.943 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 3.4 M 1,6-hexanediol, 0.1 M Tris and 0.2 M MgCl2 (H2O)6 |