6DQW
Flavobacterium johnsoniae class Id ribonucleotide reductase alpha subuint
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-12 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.97872 |
Spacegroup name | P 61 |
Unit cell lengths | 161.031, 161.031, 187.733 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.530 - 2.600 |
R-factor | 0.2084 |
Rwork | 0.206 |
R-free | 0.25080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wgh |
RMSD bond length | 0.006 |
RMSD bond angle | 1.037 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | BALBES |
Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.640 |
High resolution limit [Å] | 2.600 | 7.050 | 2.600 |
Rmerge | 0.100 | 0.026 | 0.905 |
Rmeas | 0.104 | 0.027 | 0.946 |
Rpim | 0.031 | 0.008 | 0.276 |
Number of reflections | 85045 | 4336 | 4260 |
<I/σ(I)> | 8 | ||
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 11.6 | 11.3 | 11.6 |
CC(1/2) | 0.999 | 0.856 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.25 M magnesium chloride, 0.1 M Tris pH 7.0, 10% (w/v) PEG 8000 |