6DQT
Crystal structure of Haemophilus influenzae OppA complex with LGG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-21 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.0781 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.522, 90.953, 108.860 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.630 - 1.950 |
R-factor | 0.1683 |
Rwork | 0.166 |
R-free | 0.20490 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.410 |
Data reduction software | XDS (Jan 26, 2018) |
Data scaling software | Aimless (0.6.3) |
Phasing software | BALBES (1.0.0) |
Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.630 | 2.000 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.091 | 0.665 |
Number of reflections | 36506 | 2548 |
<I/σ(I)> | 15.3 | 3.7 |
Completeness [%] | 99.6 | 99.8 |
Redundancy | 10.9 | 11 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.1M sodium acetate pH 4.6, 2.3M ammonium sulfate |