6DQP
Crystal structure of SsuE FMN reductase Delta118 mutant in apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.918, 41.812, 182.256 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.807 - 1.546 |
R-factor | 0.1612 |
Rwork | 0.160 |
R-free | 0.17840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4pty |
RMSD bond length | 0.009 |
RMSD bond angle | 1.032 |
Data reduction software | XDS |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.807 | 1.580 |
High resolution limit [Å] | 1.546 | 1.546 |
Number of reflections | 46844 | |
<I/σ(I)> | 19.3 | |
Completeness [%] | 99.9 | |
Redundancy | 8.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 100 mM CHES: NaOH pH 9.5, 30 % (w/v) PEG 3000 |