6DQG
Human glutamate dehydrogenase, H454Y mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-12-10 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5406 |
| Spacegroup name | P 1 |
| Unit cell lengths | 96.803, 98.380, 124.300 |
| Unit cell angles | 85.94, 69.35, 61.00 |
Refinement procedure
| Resolution | 29.775 - 2.700 |
| R-factor | 0.2425 |
| Rwork | 0.241 |
| R-free | 0.27870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.949 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.775 | |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 98035 | |
| <I/σ(I)> | 12.9 | |
| Completeness [%] | 95.9 | |
| Redundancy | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 295 | Reservoir: 10% w/v PEG8000, 0.1 M sodium chloride, 1.3% w/v octyl-b-glucopyranoside, 7.5% v/v MPD, 0.1 M sodium phosphate, pH 6.8, 1 mM sodium azide |
