6DPJ
Crystal Structure of Bacillus Halodurans Ribonuclease H1 K196A in Complex with an RNA/DNA Hybrid: Reaction in 4 mM Mn2+ and 200 mM K+ for 80 s at 21 C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 81.884, 37.549, 62.400 |
| Unit cell angles | 90.00, 97.23, 90.00 |
Refinement procedure
| Resolution | 19.409 - 1.550 |
| R-factor | 0.1716 |
| Rwork | 0.170 |
| R-free | 0.21070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdbid 1ZBL |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.025 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless (0.5.31) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 19.410 | 19.410 | 1.580 |
| High resolution limit [Å] | 1.550 | 8.490 | 1.550 |
| Rmerge | 0.072 | 0.050 | 0.663 |
| Rmeas | 0.101 | 0.070 | 0.937 |
| Rpim | 0.072 | 0.050 | 0.663 |
| Total number of observations | 42404 | ||
| Number of reflections | 26181 | 160 | 666 |
| <I/σ(I)> | 4.4 | ||
| Completeness [%] | 94.8 | 82.4 | 49.7 |
| Redundancy | 1.6 | 1.6 | 1.4 |
| CC(1/2) | 0.989 | 0.985 | 0.407 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2 |
