6DPC

Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 12 mM Mn2+ and 200 mM K+ for 40 s at 21 C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-BM-B
Synchrotron site	APS
Beamline	23-BM-B
Temperature [K]	100
Detector technology	CCD
Collection date	2016-12-16
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	81.821, 37.426, 61.946
Unit cell angles	90.00, 96.83, 90.00

Refinement procedure

Resolution	20.010 - 1.340
R-factor	0.15
Rwork	0.149
R-free	0.18000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zbl
RMSD bond length	0.007
RMSD bond angle	1.061
Data reduction software	HKL-2000
Data scaling software	Aimless (0.5.31)
Phasing software	PHENIX
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.010	1.360
High resolution limit [Å]	1.340	1.340
Total number of observations	38831
Number of reflections	38831	1857
<I/σ(I)>	7
Completeness [%]	92.8	89.9
Redundancy	1	1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2