6DP7

Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 500 mM Mn2+ and 200 mM K+ for 40 s at 21 C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-BM-B
Synchrotron site	APS
Beamline	23-BM-B
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-25
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	82.013, 37.557, 61.932
Unit cell angles	90.00, 96.79, 90.00

Refinement procedure

Resolution	17.983 - 1.381
R-factor	0.1698
Rwork	0.169
R-free	0.19230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zbl
RMSD bond length	0.007
RMSD bond angle	0.946
Data reduction software	HKL-2000
Data scaling software	Aimless (0.5.31)
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	17.980	1.400
High resolution limit [Å]	1.380	1.380
Rmerge	0.061	0.296
Rmeas	0.086	0.419
Rpim	0.061	0.296
Total number of observations	74048	2990
Number of reflections	38088	1523
<I/σ(I)>	10.1	4.1
Completeness [%]	98.3	76.4
Redundancy	1.9	2
CC(1/2)	0.985	0.763

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2