6DP2
Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 7.5 mM Mg2+ and 75 mM K+ for 40 s at 21 C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-23 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 81.396, 37.309, 62.340 |
| Unit cell angles | 90.00, 96.62, 90.00 |
Refinement procedure
| Resolution | 20.020 - 1.662 |
| R-factor | 0.1593 |
| Rwork | 0.157 |
| R-free | 0.19610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zbl |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.038 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless (0.5.31) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.020 | 20.020 | 1.690 |
| High resolution limit [Å] | 1.660 | 8.950 | 1.660 |
| Total number of observations | 21230 | ||
| Number of reflections | 21230 | 140 | 887 |
| <I/σ(I)> | 6.9 | ||
| Completeness [%] | 96.0 | 90.3 | 76.5 |
| Redundancy | 1 | 1 | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2 |
