6DOL

Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 25 mM K+ for 120 s at 21 C (dataset 2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2016-02-15
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	81.801, 37.113, 62.105
Unit cell angles	90.00, 96.44, 90.00

Refinement procedure

Resolution	20.060 - 1.433
R-factor	0.1531
Rwork	0.151
R-free	0.18500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zbl
RMSD bond length	0.007
RMSD bond angle	1.002
Data reduction software	HKL-2000
Data scaling software	Aimless (0.5.31)
Phasing software	PHENIX
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.060	20.060	1.460
High resolution limit [Å]	1.430	7.850	1.430
Total number of observations	33645
Number of reflections	33645	208	1327
<I/σ(I)>	5.7
Completeness [%]	98.2	90.2	79.6
Redundancy	1	1	1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	14% PEG3350, 20% glycerol, 200 mM potassium iodide, 5 mM calcium chloride