6DOB
Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 200 mM K+ for 200 s at 21 C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-07-28 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 81.376, 38.094, 62.117 |
Unit cell angles | 90.00, 96.16, 90.00 |
Refinement procedure
Resolution | 32.289 - 1.343 |
R-factor | 0.1701 |
Rwork | 0.169 |
R-free | 0.19580 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.116 |
Data reduction software | HKL-2000 |
Data scaling software | Aimless (0.5.31) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 32.290 | 32.290 | 1.370 |
High resolution limit [Å] | 1.340 | 7.360 | 1.340 |
Total number of observations | 40966 | ||
Number of reflections | 40966 | 231 | 1634 |
<I/σ(I)> | 7.1 | ||
Completeness [%] | 96.7 | 82.7 | 78.3 |
Redundancy | 1 | 1 | 1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 294 | 14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2 |