6DOA

Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 200 mM K+ for 480 s at 21 C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-BM-B
Synchrotron site	APS
Beamline	23-BM-B
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-28
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	81.731, 37.890, 62.695
Unit cell angles	90.00, 96.66, 90.00

Refinement procedure

Resolution	31.136 - 1.474
R-factor	0.161
Rwork	0.160
R-free	0.18600
RMSD bond length	0.009
RMSD bond angle	1.156
Data reduction software	HKL-2000
Data scaling software	Aimless (0.5.31)
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	31.140	31.140	1.500
High resolution limit [Å]	1.470	8.070	1.470
Total number of observations	32242	191	1540
Number of reflections	32242	191	1540
<I/σ(I)>	9.4	24.8	1.4
Completeness [%]	99.4	88.5	94.9
Redundancy	1	1	1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2