6DO9

Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 200 mM K+ for 120 s at 21 C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-14
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	81.301, 37.773, 62.509
Unit cell angles	90.00, 96.22, 90.00

Refinement procedure

Resolution	19.859 - 1.360
R-factor	0.1545
Rwork	0.153
R-free	0.17540
RMSD bond length	0.009
RMSD bond angle	1.276
Data reduction software	HKL-2000
Data scaling software	Aimless (0.5.31)
Phasing software	PHENIX
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.860	19.860	1.380
High resolution limit [Å]	1.360	7.440	1.360
Total number of observations	40176
Number of reflections	40176	252	1616
<I/σ(I)>	6.6
Completeness [%]	98.2	92.7	82
Redundancy	1	1	1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2