6DNW
Sequence Requirements of the Listeria innocua prophage attP site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-11-06 |
Detector | DECTRIS PILATUS 300K |
Wavelength(s) | 0.97919 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.291, 57.112, 167.883 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.971 - 2.849 |
R-factor | 0.2213 |
Rwork | 0.219 |
R-free | 0.25600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4kis |
RMSD bond length | 0.003 |
RMSD bond angle | 0.563 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.971 | 2.950 |
High resolution limit [Å] | 2.849 | 2.850 |
Rmerge | 0.057 | 0.414 |
Number of reflections | 11185 | 1082 |
<I/σ(I)> | 14.9 | 2.3 |
Completeness [%] | 94.1 | 98.7 |
Redundancy | 3 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294.15 | Crystals were obtained by hanging drop vapor diffusion at 21oC in 100 mM Tris HCL pH 7.5, 200 mM ammonium sulfate, and 25% (w:v) polyethylene glycol 3350 |