6DNS

Endo-fucoidan hydrolase MfFcnA9 from glycoside hydrolase family 107

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-20
Detector	DECTRIS PILATUS3 R 200K-A
Wavelength(s)	1.54178
Spacegroup name	P 43 21 2
Unit cell lengths	61.914, 61.914, 324.395
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.240
R-factor	0.2541
Rwork	0.253
R-free	0.27100
RMSD bond length	0.007
RMSD bond angle	1.013
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	2.290
High resolution limit [Å]	2.240	6.050	2.240
Rmerge	0.152	0.111	0.217
Rmeas	0.168	0.118	0.257
Rpim	0.068	0.039	0.135
Number of reflections	31364	1788	1517
<I/σ(I)>	3.6
Completeness [%]	99.6	99	99.9
Redundancy	4.9	9.2	3.4
CC(1/2)		0.990	0.862

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	0.2 M MgCl2, 0.1 M Bis-Tris (pH5.6), 19% PEG 3350