6DNR
PRPP Riboswitch bound to PRPP, ligand-free structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-27 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 63.773, 63.773, 376.493 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.807 - 2.896 |
R-factor | 0.2185 |
Rwork | 0.212 |
R-free | 0.27340 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.241 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.950 |
High resolution limit [Å] | 2.890 | 2.900 |
Rmerge | 0.154 | 1.554 |
Rpim | 0.051 | 0.388 |
Number of reflections | 10251 | |
<I/σ(I)> | 27.6 | |
Completeness [%] | 93.8 | |
Redundancy | 9.5 | |
CC(1/2) | 0.834 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 M NH4-acetate, 0.1 M HEPES-KOH pH 7.4, and 45% 2-methyl-2,4-pentanediol (v/v) |