6DNO

Crystal structure of Protein Phosphatase 1 (PP1) bound to the muscle glycogen-targeting subunit (Gm)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97945
Spacegroup name	C 2 2 21
Unit cell lengths	60.476, 65.744, 160.355
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.900 - 1.450
R-factor	0.187
Rwork	0.186
R-free	0.20960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mov
RMSD bond length	0.005
RMSD bond angle	0.963
Data reduction software	XDS (oct 15, 2015)
Data scaling software	Aimless (0.5.17)
Phasing software	PHENIX (2.5.6)
Refinement software	PHENIX ((1.13_2998))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.900	1.470
High resolution limit [Å]	1.450	1.450
Rmerge	0.061	1.147
Number of reflections	56091
<I/σ(I)>	12.2	0.9
Completeness [%]	98.5	72.9
Redundancy	5.1	2.9
CC(1/2)	0.998	0.337

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.6	277	0.4 M magnesium formate dihydrate, 0.1 M Sodium Acetate trihydrate