6DN6
SPRY domain-containing SOCS box protein 2 complexed with INNN(ABU) cyclic peptide inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-06-29 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.95370 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.040, 61.950, 84.930 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.460 - 1.590 |
R-factor | 0.175 |
Rwork | 0.174 |
R-free | 0.20200 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.000 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.500 | 1.680 |
High resolution limit [Å] | 1.590 | 1.590 |
Rmerge | 0.074 | 0.743 |
Rpim | 0.024 | 0.241 |
Number of reflections | 29881 | |
<I/σ(I)> | 22.6 | 3.2 |
Completeness [%] | 99.9 | |
Redundancy | 11.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 277.15 | 0.1M CHES, 20% PEG 8,000 |