6DN2
CRYSTAL STRUCTURE OF THE FMN RIBOSWITCH BOUND TO BRX1354 SPLIT RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-05-31 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 71.070, 71.070, 140.480 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.550 - 2.880 |
| R-factor | 0.202 |
| Rwork | 0.199 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f4e |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.710 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.230 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.085 | 0.266 |
| Number of reflections | 8864 | |
| <I/σ(I)> | 9.14 | 2.52 |
| Completeness [%] | 83.4 | 54.2 |
| Redundancy | 7.1 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6.5 | 293.15 | PEG 4000 |
