6DMX
HBZ56 in complex with KIX and c-Myb
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-16 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.997, 80.311, 64.641 |
| Unit cell angles | 90.00, 92.60, 90.00 |
Refinement procedure
| Resolution | 40.970 - 2.800 |
| R-factor | 0.24198 |
| Rwork | 0.239 |
| R-free | 0.30083 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2agh |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.056 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 13196 | |
| <I/σ(I)> | 16.6 | |
| Completeness [%] | 95.2 | |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG 3350, ammonium iodide |
