6DM6
Structure of DNA polymerase III subunit beta from Rickettsia conorii in complex with a natural product
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-08-18 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97872 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 83.860, 41.830, 127.400 |
Unit cell angles | 90.00, 93.92, 90.00 |
Refinement procedure
Resolution | 49.017 - 2.250 |
R-factor | 0.181 |
Rwork | 0.178 |
R-free | 0.23480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6dlk |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (dev_3126) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.017 | 49.017 | 2.310 |
High resolution limit [Å] | 2.250 | 10.060 | 2.250 |
Rmerge | 0.063 | 0.018 | 0.552 |
Rmeas | 0.074 | 0.021 | 0.645 |
Total number of observations | 158384 | ||
Number of reflections | 42516 | 518 | 3164 |
<I/σ(I)> | 15.54 | 48.36 | 2.39 |
Completeness [%] | 99.6 | 95.6 | 99.8 |
Redundancy | 3.725 | 3.203 | 3.775 |
CC(1/2) | 0.999 | 1.000 | 0.871 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 290 | Rigaku Wizard Cryo 1/2 A8: 0.1M Sodium cacodylate pH6.5, 35% 2-ethoxyethanol 0.4:0.4uL drops, direct cryo: tray 290984a8: puck mbp2-7 |