6DM6

Structure of DNA polymerase III subunit beta from Rickettsia conorii in complex with a natural product

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2017-08-18
Detector	RAYONIX MX-300
Wavelength(s)	0.97872
Spacegroup name	P 1 21 1
Unit cell lengths	83.860, 41.830, 127.400
Unit cell angles	90.00, 93.92, 90.00

Refinement procedure

Resolution	49.017 - 2.250
R-factor	0.181
Rwork	0.178
R-free	0.23480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6dlk
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev_3126)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.017	49.017	2.310
High resolution limit [Å]	2.250	10.060	2.250
Rmerge	0.063	0.018	0.552
Rmeas	0.074	0.021	0.645
Total number of observations	158384
Number of reflections	42516	518	3164
<I/σ(I)>	15.54	48.36	2.39
Completeness [%]	99.6	95.6	99.8
Redundancy	3.725	3.203	3.775
CC(1/2)	0.999	1.000	0.871

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	290	Rigaku Wizard Cryo 1/2 A8: 0.1M Sodium cacodylate pH6.5, 35% 2-ethoxyethanol 0.4:0.4uL drops, direct cryo: tray 290984a8: puck mbp2-7