6DM3
Crystal structure of the SH2 domain from RavO (Lpg1129) from Legionella pneumophila, apoprotein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97924 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 50.037, 72.981, 71.961 |
| Unit cell angles | 90.00, 109.89, 90.00 |
Refinement procedure
| Resolution | 24.810 - 1.950 |
| R-factor | 0.2638 |
| Rwork | 0.261 |
| R-free | 0.31790 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.224 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((dev_3092: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.058 | 0.414 |
| Rmeas | 0.071 | 0.517 |
| Rpim | 0.040 | 0.303 |
| Number of reflections | 16407 | 823 |
| <I/σ(I)> | 28.56 | |
| Completeness [%] | 92.4 | 95.8 |
| Redundancy | 2.8 | 2.6 |
| CC(1/2) | 0.928 | 0.928 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.2 M potassium chloride, 20% (w/v) PEG 5K MME |
