6DLT

PRPP Riboswitch bound to PRPP, native structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	83.567, 90.756, 74.174
Unit cell angles	90.00, 122.00, 90.00

Refinement procedure

Resolution	20.000 - 2.900
R-factor	0.22548
Rwork	0.225
R-free	0.24349
RMSD bond length	0.005
RMSD bond angle	1.237
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.156	0.632
Rpim	0.081	0.298
Number of reflections	9366
<I/σ(I)>	15.7
Completeness [%]	92.4
Redundancy	5.1
CC(1/2)	0.881

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2 M NH4-acetate, 0.1 M HEPES-KOH pH 7.4, and 45% 2-methyl-2,4-pentanediol (v/v)